Isolation and Identification of L-asparaginase Producing Bacteria from Soils of Different Agroclimatic Zones of Jammu (J&K), India

A milestone was set in the field of medicine with discovery L-asparaginase present serum guinea pig responsible for action against acute lymphoblastic leukemia. Since then, use as a potential anti-cancer drug has been great success modern oncology. Despite its varied sources, new sources are continuously being explored to obtain high therapeutic index drug. The study aims isolate producing microorganisms from environmental niches Northwestern Himalayas. climatic conditions region and ancient agricultural practices which still followed these areas, add up diverse microbial repository habitat can be potent asparaginase source. In study, soil samples different regions Jammu were collected aseptically. From isolation studies, total 44 bacterial isolates obtained. Pure cultures then screened activity both qualitatively well quantitatively using agar plate nesselerization method. Screening studies resulted producer isolated black gram sample Rajouri, showing an enzyme 9.14 U/ml. Based on biochemical molecular approaches, identified Enterobacter aesburiae strain R16C1 / No. MT93543. pure culture soils Himalayas possessing activity. Further, optimization help considerably increasing tested human cancer lines efficacy.